Molecular Toolkit Index Toolkit Help

Reverse Translate a Protein


Overview
Enter a protein sequence and obtain its reverse translation for use in design of degenerate oligonucleotides. The program uses the standard genetic code and does not consider differences in codon usage.
Instructions
Enter or paste an amino acid sequence (single letter codes only) into the upper text box, then click the Reverse Translate button. The reverse translation is placed into the lower text box.

For each amino acid in the sequence, the possible nucleotides at each position in the codon are displayed. For example, there are six codons for leucine (CTT, CTC, CTA, CTG, TTA and TTG), and thus, the base at position 1 can be a C or T, position 2 is always a T, and the base at position 3 can be T, C, A or G. This pattern can be depicted as seen to the right.
Leucine codons:

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Last updated on June 20, 1998
Please send comments and report any bugs, errors or inaccuracies to: rbowen@lamar.colostate.edu