Cutting and pasting are two of the first skills children learn, and the tools they use are scissors and glue. Similarly, cutting DNA and pasting DNA fragments together typically are among the first techniques learned in the molecular biology lab and are fundamental to all recombinant DNA work. Such manipulations of DNA are conducted by a toolkit of enzymes: restriction endonucleases are used as molecular scissors, DNA ligase functions to bond pieces of DNA together, and a variety of additional enzymes that modify DNA are used to facilitate the process.
Core concepts related to restriction endonucleases and other DNA modifying enzymes are presented as the following topics:
Advanced and supplemental topics:
- Restriction Enzymes
- Other DNA Modifying Enzymes